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Longevity

Methylene blue: the research history.

Methylene blue is listed in 5 mg and 10 mg options. Its research spans many applications, so an imaging result needs to retain its own regimen and follow-up period.

27,299records matching the ingredient search
1connected formulations
6displayed publication records

How the research connects to the formula

The displayed 2016 randomized study enrolled twenty-six people and measured functional MRI and task performance before and after administration. It examined a short observation period rather than a long-term clinical outcome.

The numerical strength on the product page and the regimen administered in a study are separate fields. Keeping both prevents a finding from a different dose or formulation from being assigned to the listed options.

Current HealthRX.com formulation map
ProductListed formulationConnected ingredient research
Methylene BlueMethylene Blue 5 / 10 mgMethylene blue

Publication history

When the ingredient appeared in the literature

The chart counts records mentioning the search terms in their title or abstract. It includes research beyond longevity. Use the period controls to inspect the count.

2026: 1,312 indexed publications. 2026 includes publications dated through October 5. The earlier bars cover longer periods than the annual bars.

View the chart as a data table
PeriodPublications
1900-1959357
1960-1969260
1970-1979584
1980-19891200
1990-19992810
2000-20093304
2010-20142858
2015-20194860
20201399
20211469
20221671
20231748
20241728
20251739
20261312
Search query and data source

Source: Europe PMC’s PubMed-indexed records. Retrieved October 5, 2026. The index’s firstPublicationDate field supplies the dates and chart periods; it can normalize a month-only record to the first day of the month. Dates through October 5 are included. Counts can change as the index adds or corrects records.

TITLE_ABS:("methylene blue") AND SRC:MED AND FIRST_PDATE:[1900-01-01 TO 2026-10-05]

Open this literature search · Download the data

Dated publications

Read individual records in context

The timeline combines selected references with a sample from the ingredient search. Dates use the index’s firstPublicationDate field; the PubMed record may distinguish an earlier online date from the print date. Written study notes appear alongside the records that have them. Other entries show the indexed bibliography and link to the paper.

research-article · Randomized Controlled Trial · Journal Article

Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain.

Rodriguez P, Zhou W, Barrett DW, Altmeyer W, Gutierrez JE, Li J, Lancaster JL, Gonzalez-Lima F, Duong TQ. Radiology. PMID 27351678.

Study design
Randomized human imaging study
What was tested
Methylene blue and functional MRI measures.
What was reported
The researchers measured brain imaging and task performance.
How it connects to the catalog
An imaging study is different from evidence of lasting clinical benefit. The administered regimen must also be compared with the strength and formulation being considered.
Read the PubMed record · Publisher record

Study comparison

PublicationDesign and regimenReported findingInterpretation
2016-06-28: Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain.Randomized human imaging study. Methylene blue and functional MRI measures.The researchers measured brain imaging and task performance.An imaging study is different from evidence of lasting clinical benefit. The administered regimen must also be compared with the strength and formulation being considered.

Questions that shape the comparison

What administered dose did the source use? Was its endpoint an imaging signal, task performance or a sustained clinical change?

Source records

Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain.Rodriguez P, Zhou W, Barrett DW, Altmeyer W, Gutierrez JE, Li J, Lancaster JL, Gonzalez-Lima F, Duong TQ.Radiology. 2016-06-28. PMID 27351678. research-article, Randomized Controlled Trial, Journal Article.
Comparison of compound methylene blue (methylene blue + ropivacaine) and bupivacaine liposome for pain control after mixed hemorrhoid surgery: A comprehensive analysis based on clinical studies.Mai G, Yu J, Li J.The Journal of international medical research. 2026-07-10. PMID 42427264. Comparative Study, research-article, Journal Article, Observational Study.
Contrast-Enhanced Ultrasound Combined with Methylene Blue for Sentinel Lymph Node Biopsy After Neoadjuvant Chemotherapy in Patients with Initially cN1 Breast Cancer: A Multicenter Prospective Cohort Study.Zhai D, Tang H, Fan Y, AiErken N, Zhang M, Shi Y, Zhang Y, Zheng Y, Shan Z, Lin Y.Cancers. 2026-07-24. PMID 42588603. research-article, Journal Article.
Comparative margins between colorectal carcinoma and peritumoral injected methylene blue.Cardoso PO, Petroianu A.Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery. 2026-08-21. PMID 42659516. Comparative Study, research-article, Journal Article.
Methylene Blue Oral Rinse for Cancer-Related Oral Mucositis Pain #540.S Carlson E, Arnold R.Journal of palliative medicine. 2026-09-10. PMID 42722988. Journal Article.
Evaluation of methylene blue and tissue marker for assessing sciatic nerve staining one hour after ultrasound-guided injection in rat cadavers.Castro D, Martinez V, Escobar A.Veterinary anaesthesia and analgesia. 2026-09-17. PMID 42753629. Journal Article.

Research data assembled by HealthRX.com from the displayed source records, October 5, 2026. The atlas contains a dated literature search and selected studies; it is not an exhaustive systematic review.